Upload a CSV file generated from the Cary 3E:

Zero (baseline) spectra at nm.

Correct for the following changes in volume: , with an initial volume of .

(For example: '10 of 1' means that you added 10 aliquots of 1 μL each.)

Normalize spectra at nm - (Use with caution).

Calculate difference spectra.

Smooth spectra using a window of nm.

Calculate a binding curve using a peak wavelength of nm and a trough wavelength of nm, with the following titrant concentrations: .

(For example: '5 of 2.5 and 5 of 5' means that the substrate concentration increased 2.5 μM for the first five aliquots and 5 μM for the next five aliquots.)