Upload a CSV file generated from the Cary 3E:
Zero (baseline) spectra at nm.
Correct for the following changes in volume: , with an initial volume of .
(For example: '10 of 1' means that you added 10 aliquots of 1 μL each.)
Normalize spectra at nm - (Use with caution).
Calculate difference spectra.
Smooth spectra using a window of nm.
Calculate a binding curve using a peak wavelength of nm and a trough wavelength of nm, with the following titrant concentrations: .
(For example: '5 of 2.5 and 5 of 5' means that the substrate concentration increased 2.5 μM for the first five aliquots and 5 μM for the next five aliquots.)
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